Separation of cell components in the zonal ultracentrifuge.
نویسندگان
چکیده
Zonal separation of particles in density gradients contained in hollow or tubeless rotors has been explored with the aim of developing a preparative counterpart of the analytical ultracentrifuge. A rotor with a capacity of 1625 milliliters has been tested up to 22,500 revolutions per minute, with sucrose density gradients used. Excellent separation of subcellular particles has been achieved as well as partial separation of the albumin and globulin peaks of serum.
منابع مشابه
STUDIES ON ISOLATED CELL COMPONENTS XVII. The Distribution of Cytochrome Oxidase Activity in Rat Liver Brei Fractionated in the Zonal Ultracentrifuge
The zonal ultracentrifuge was used to separate the subcellular components of rat liver brei into soluble phase, microsomal, mitochondrial, membranous fragments, and nuclear fractions during a single centrifugafion. The centrifuge was run at 10,000 to 30,000 RPM for 15 to 240 minutes, and the rotor contained a 1200 ml sucrose gradient, varying linearly with radius from 17 to 55 per cent sucrose ...
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The zonal ultracentrifuge was used to separate the subcellular components of rat liver brei into soluble phase, microsomal, mitochondrial, membranous fragments, and nuclear fractions during a single centrifugation. The centrifuge was run at 10,000 to 30,000 RPM for 15 to 240 minutes, and the rotor contained a 1200 ml sucrose gradient, varying linearly with radius from 17 to 55 per cent sucrose ...
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عنوان ژورنال:
- Science
دوره 136 3516 شماره
صفحات -
تاریخ انتشار 1962